Barrow bowed out of the Carabao Cup following a 1-0 defeat against Bolton Wanderers at the Toughsheet Community Stadium.
A single strike from Zac Ashworth settled the contest after his well-worked free-kick found the bottom corner.
Pete Wild handed full competitive debuts to Courtney Duffus and Junior Tiensia, who were both introduced from the bench against Tranmere Rovers last weekend.
As expected, the hosts started the game the stronger side trying to control the possession, meaning high defensive standards would need to be upheld for Wild's men.
Soon after the whistle was blown, Bolton fashioned their first chance of the evening. Dan Nlundulu held the ball up well in the penalty area and found space to turn and shoot before he fired into the path of Paul Farman, who spread himself well to push the ball away.
Soon after, Paris Maghoma proved difficult to deal with, as he burst down the right flank. The 22-year-old eventually squared to Carlos Mendes Gomes, who failed to trouble Farman.
The Brentford loanee caused problems once more on the half-hour mark. This time he operated on the left, skipping past three players before curling a shot beyond the far post.
The Bluebirds then manufactured a chance of their own, coming tantalisingly close to opening the scoring. A cross from the right-hand side was cleared as far as Tiensia, who rifled the ball into the box, finding Duffus, who deflected narrowly past the upright.
Despite Wild’s men having grown into the game, it was the Trotters that broke the deadlock. A mischievous free kick from Ashworth deceived Farman, giving Bolton the lead.
The last action of the opening 45 saw Barrow produce another opportunity, this time from a set piece. A well-worked corner routine saw the ball played short and then driven to Robbie Gotts, who failed to keep his effort down.
After the interval, the game quietened, and chances became scarce. The visitors defended well, to ensure that the advantage was not immediately built upon.
New boy Tiensia threatened shortly after the hour mark, marauding down the touchline. The 22-year-old found his way into the penalty area and was unfortunate not to test Joel Coleman in the Bolton goal.
At the other end, Wild’s backline continued to stand firm. George Ray kept his side in the game, covering ground to prevent substitute Victor Adeboyejo from getting a shot away.
Another chance fell to Ian Evatt’s side as injury time loomed. Morley played the ball into Adeboyejo, but Farman was on hand, once again, to thwart the Bolton forwards.
Soon after substitute Elliot Newby did well to create space. His looping cross fell to David Worrall, who mistimed his audacious swing.
Foley then collected in the deep, swung another ball into the box, and this time it stuck. Ray played to captain Niall Canavan, who struggled to adjust. Despite the pressure, the tie ended in defeat.
Barrow is back in League Two action on Saturday, welcoming Sutton United to the SO Legal Stadium.
Barrow lineup – Farman; Warren, Feely (Worrall, 80), Ray, Canavan, Tiensia (Newby, 80); Foley, White (Spence, 65), Campbell (Garner, 65); Gottts; Duffus (Acquah, 80).